Development of efficient peptide drug discovery system by using highly specific tag-mediated protein immobilization technique

我们试图开发一种高效的肽类药物发现系统，利用高度特异性的标签介导的蛋白质固定化技术。以FOXP3和NFkB（p50）两种癌症相关蛋白为靶点，从T7噬菌体随机肽库中筛选位点特异性抑制肽。发现候选肽的有效分离与噬菌体文库预处理与固体底物固定蛋白之间存在明显的相关性。此外，还成功获得了一些与靶蛋白具有高结合亲和力的功能性抑制肽。

We have tried to develop an efficient peptide drug discovery system utilizing highly specific tag-mediated protein immobilization technique. Two cancer-related proteins, FOXP3 and NFkB(p50), were adopted as targets and site specific inhibitory peptides were screened from T7 phage random peptide library. The clear correlation between effective isolation of candidate peptides and pretreatment of phage library with solid substrate for protein immobilization was found. In addition, a number of functional inhibitory peptides with high binding affinity with target proteins were successfully obtained.