Analyses of functional RNAs and target genes involves in leg regeneration using transgenic cricket, Gryllus bimaculatus

我们从胚胎和再生组织的文库中克隆了几种miRNAs，并观察了这些miRNAs在胚胎中的表达。我们揭示了克隆的miRNAs的表达模式。在我们的miRNAs文库中发现了Wnt组分基因的靶基因miR-8。此外，为了分析miRNA及其靶基因的功能，我们利用转基因蟋蟀建立了细胞轨迹分析。此外，我们建立了使用锌指核酸酶（ZFN）和转录激活因子样（TAL）效应物核酸酶（TALEN）的基因靶向系统。利用这些系统，我们现在正试图研究参与板球腿再生的miRNA的功能。

We cloned several miRNAs from libraries of embryonic and regenerating tissues and observed these expressions in the embryos. We revealed expression patterns of cloned miRNAs. We found miR-8, which is a target of Wnt component gene in our miRNAs libraries. Moreover, to analyze the function of miRNA and its target genes, we established cell trajectory analysis using the transgenic cricket. Furthermore, we established gene-targeting systems using zinc finger nucleases(ZFNs) and transcription activator-like(TAL) effector nucleases(TALENs). Using these systems, we are now trying to investigate the function of the miRNA involves in the leg regeneration in cricket.