Synthesis of Cardiolipin Analogs and Characterization of Their Effects on Induction of the Peroxidase Activity of Cytochrome c

心磷脂(CL)是哺乳动物线粒体中发现的一种主要磷脂，具有多种生物学功能。细胞色素c(Cytc)过氧化物酶活性被CL和过氧化氢激活被认为是早期细胞凋亡的关键事件。我们开发了一种简明的CL合成方法。在手头有大量CL的情况下，我们研究了脂质体系中细胞色素c-CL复合体的形成和过氧化物酶活性诱导所需的CL的结构因素。结果表明，在较低的CL含量下，CL的饱和酰链有利于CL结合的细胞色素c过氧化物酶活性的激活，而双键的关键作用并不是细胞色素c-CL相互作用的一般特征。

Cardiolipin(CL) is a major phospholipid found in mammalian mitochondria with a multitude of biological functions. Activation of the peroxidase activity of cytochrome c(cyt c) by CL and hydrogen peroxide in mitochondria has been suggested to be a key event in early apoptosis stage. We have developed the concise procedure for the synthesis of CL. With numerous CLs in hand, we examined the structural factors of CL required for the formation and the induction of peroxidase activity of cyt c-CL complex in liposomal system. The activation efficiencies of CLs are well correlated with the binding affinities to cyt c. Our results revealed that at low CL content, the saturated acyl chain of CL is favorable for the activation of peroxidase activity of CL-bound cyt c and the proposed critical role of double bond in the acyl chains is not a general feature of the cyt c-CL interaction.