Investigation of manganese transport system in neuronal cells

锰（Mn）是一种必需元素，但暴露于过量的Mn会导致类似帕金森病的症状。虽然几种转运蛋白可能参与锰的吸收，只有DMT 1和转铁蛋白受体的作用已被检查关于锰在神经系统中的运输。为了证实DMT 1的贡献，我们首先检查了DMT 1 siRNA的作用。结果表明，DMT 1的siRNA能抑制人神经母细胞瘤SH-SY 5 Y细胞对Mn的摄取。在SH-SY 5 Y和小鼠海马HT 22细胞中，ZIP 14的siRNA的引入抑制了Mn^<2+>和Cd^<2+>的摄取，但ZIP 8没有抑制Mn ^<2+>和Cd^<2+>的摄取。这些数据表明，ZIP 14除了DMT 1可能发挥重要作用，在神经元细胞中的锰运输。

Manganese(Mn) is an essential element, but exposure to excess amount of Mn causes symptoms similar to Parkinson's disease. Although several transporters may be involved in Mn uptake, only the role of DMT1 and transferrin receptor have been examined regarding Mn transport in nervous systems. To confirm the contribution of DMT1, we first examined the effects of DMT1 siRNA. As a result, the uptake of Mn in human neuroblastoma SH-SY5Y cells was suppressed by introduction of siRNA of DMT1.Next, we focused on zinc transporters, ZIP8 and ZIP14, which can transport Mn as well as Zn and Cd. The uptakes of Mn^<2+> and Cd^<2+> were suppressed by the introduction of siRNA of ZIP14, but not by ZIP8, in both SH-SY5Y and mouse hippocampus HT22 cells. These data suggest that ZIP14 in addition to DMT1 might play an important role in Mn transport in neuronal cells.