Gene therapy for myocardial infarction by using lentivirus encoding kit ligand

本研究的目的是评价KIT配体(KL)亚型抗心肌梗死(MI)的作用。Western blotting和流式细胞仪检测证实KL在转导细胞中表达。观察内源性KL-1、KL-2和c-kit在小鼠心肌梗死后的表达。我们发现心肌梗死后小鼠心脏中KL-2的表达减少。因此，使用编码KL-2的慢病毒基因治疗可能改善MI后的预后。我们本应在今年完成体内实验，但我们在制造大规模病毒方面遇到了困难。我们将继续进行活体实验。

The purpose of this study is to evaluate the effect of kit ligand(KL) isoform against myocardial infarction(MI). The KL expression in transduced cells was confirmed by western blotting and flow cytometry. Endogenous KL-1, KL-2 and c-kit expression in infarcted mouse hearts were evaluated. We found that KL-2 expression in mouse hearts was diminished after MI. Therefor gene therapy using lentivirus encoding KL-2 may improve the prognosis after MI. We were supposed to finish in vivo experiments in this year, however we have difficulties in making large scale virus. We will continue to do in vivo experiments.