Development of receptor targeting DDS of oral cancer using the nanosize particle

在本研究中，我们开发了可以识别纳米颗粒（Minicell）和EGF受体（EGFR）的双特异性抗体。首先，通过使用该抗体检测Minicell在培养的口腔癌细胞系上的表达水平，我们证实其表达与EGFR的表达成比例地增加。接下来，我们通过将抗癌药物包装的Minicell直接注射到裸鼠的肿瘤异种移植物中来测量抗肿瘤功效。结果表明，EGFR高表达肿瘤组比EGFR低表达组显示出更有效的抗肿瘤疗效。但不幸的是，结果并没有显示出统计学意义。

In present study, we developed the bispecific antibody that can recognize both nanosize particle (Minicell) and EGF receptor (EGFR). At first, expression level of Minicell on the cultured oral cancer cell line was examined by using this antibody and we confirmed that its expression was increased in proportion to that of EGFR. Next, we measured the antitumor efficacy by direct injection of anticancer drug packaged Minicell into tumour xenografts of nude mice. As a result, high EGFR expression tumor group showed more effective antitumor efficacy than low EGFR expression group. But unfortunately, the result didn’t reveal the statistical significance.