Analysis of molecular dynamics in neuronal dendrites by simultaneous multi-point fluorescence correlation spectroscopy

本研究旨在利用双光子显微镜对神经元树突胞浆中蛋白质分子的多点快速运动动力学进行测量。本人成功地从表达GFP或GFP融合的CamKII蛋白的小鼠原代培养神经元的胞质溶胶中以超过10 kHz的时间在多个点记录分辨率，并通过FCS进行分析。

This research project aimed to measure dynamics of protein molecules rapidly moving in cytosole of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz time of resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII protein was expressed, and in analyzing by FCS.