Development of a novel protein delivery system using peroxisomes

在这项研究中，我们开发了一种新型的蛋白质递送系统，分别以过氧化体和过氧化体蛋白输入系统作为蛋白质递送载体和蛋白质浓缩器。首先，我们建立了一种高纯度的过氧酶体的亲和纯化方法，在这种方法中，目的蛋白已经被高效地导入细胞内。接下来，我们开发了一种有效的方法来在纯化的过氧体表面展示特定的抗体，并利用它来依赖抗体和功能递送包裹在过氧体中的目标蛋白。最后，我们成功地证明了α-半乳糖苷酶A，一种治疗法布里病的蛋白制剂，可以通过这种新的蛋白递送系统有效地输送到靶溶酶体内。

In this study, we developed a novel protein delivery system using peroxisome and the peroxisomal protein import system as a protein delivery carrier and a protein concentrator, respectively. First, we established an affinity purification method for highly purified peroxisomes in which the target protein had been efficiently imported intracellularly. Next, we developed an efficient method to display specific antibodies onto the surface of the purified peroxisomes and utilized it to an antibody-dependent and functional delivery of the target protein encapsulated in the peroxisomes. Finally, we successfully demonstrated that α-galactosidase A, a therapeutic protein agent for Fabry disease, could be efficiently delivered into the target lysosomes by using this novel protein delivery system.