Development of the new method to control the molecular motor enzyme

基于我们的假设，即ATP合酶的活性可以通过γ亚基的两个α-螺旋的相对滑动来调节，我们打算限制两个α-螺旋的运动并研究酶活性的变化。然后，我们尝试在γ亚基中引入一种新型的人工开关，利用光响应蛋白的某个结构域来控制酶的活性。此外，还建立了高灵敏度的水相单分子水平酶活性检测系统。

Based on our hypothesis that the activity of ATP synthase can be regulated by the relative slippage of two alpha-helices of the gamma subunit, we intended to restrict the movement of two alpha-helices and studied the change in the enzyme activity. Then we tried to introduce a novel artificial switch into the gamma subunit to control the enzyme activity using a certain domain of light-responsible protein. In addition, the highly sensitive monitoring system to measure the enzyme activity at single molecule level at the aqueous phase was developed.