Development of Artificial Plant Leafs using Polymeric Nanofibers and Formulation of Sugar-Production System

提出了一种利用LED照射植物细胞进行大规模培养的新方法。分离的植物细胞分散在乙烯-乙酸乙烯酯共聚物(EVOH)溶液中。将该溶液作为纺丝液进行静电纺丝，得到了固定在EVOH纳米纤维毡上的植物细胞。电纺后证实细胞具有生物活性。将细胞分散在无糖的培养液中，在LED照射下培养一周。用液质联用仪(LC-MS)检测培养物中的葡萄糖和蔗糖。这表明植物细胞中光合作用产生的糖被分泌到细胞壁之外。另一方面，将细胞固定在EVOH纳米纤维垫上的培养物中没有检测到糖。这可能是因为(1)固定化的细胞数量较少；(2)必须使用光合作用性能较高的细胞来代替葛根细胞。

We developed a new method of mass cultivation for plant(Pueraria lobata) cell using LED irradiation. The isolated plant cells were dispersed in ethylene vinyl acetate copolymer(EVOH) solution. The solution was used as a spinning solution for electrospinning, and we obtained the plant cells immobilized on EVOH nanofiber mat. We confirmed that the cell had a bioactivity after the electrospinning.The cells were dispersed in a culture without sugar and it was cultured for a week under LED irradiation. The culture was tested by liquid chromatography mass spectrometry(LC-MS) and thus glucose and sucrose were detected. This indicates that sugar produced by photosynthesis in the plant cells was secreted to outside the cell walls. On the other hand, we could not detect sugar from the culture containing the cells immobilized on EVOH nanofiber mat. It is likely that(1) the cells immobilized were small in number ;(2) the cells with higher photosynthetic performance must be used instead of Pueraria lobata cells.