adductome analysis of human tissues

高效液相色谱和串联质谱仪揭示了在抗氧化条件下从人体尸检组织提取的DNA中至少有10个与脂质过氧化有关的修饰DNA碱基。在这10个butanone-etheno-2‘-deoxy-5-methylcytidine(BεmedC)，加合物中，有7个，即1，N6-亚乙基-2’-脱氧腺苷(εda)、丁酮-亚乙基-2‘-脱氧胞苷(B-εDC)、丁酮-亚乙基-2’-脱氧腺苷(Bεda)、庚酮-亚乙基-2‘-脱氧胞苷(HεDC)、庚酮-亚乙基-2’-脱氧腺苷(Hεda)和庚酮-亚乙基-2‘-脱氧鸟苷(HεDG)在胃粘膜中进行了定量。这些DNA加合物的图谱确定了组织的来源(中国或日本)，这意味着内收组图谱可能反映了个人环境的暴露。我们进一步针对这些修饰的碱基对修复机制进行了测试，发现TDG在修复其中一些加合物方面发挥了S的作用。

High performance liquid chromatography and tandem mass spectrometry disclosed at least 10 lipid peroxidation related modified DNA bases in the DNAs extracted under anti-oxidation condition from human autopsy tissues. Among these 10 DNA adducts, 7 of them, 1, N6-etheno-2'-deoxyadenosine(εdA), butanone-etheno-2'-deoxycytidine(BεdC), butanone-etheno-2'-deoxy-5-methylcytidine(BεmedC), butanoneetheno-2'-deoxyadenosine(BεdA), heptanone-etheno-2'-deoxycytidine(HεdC), heptanone-etheno-2'-deoxyadenosine(HεdA), and heptanone-etheno-2'-deoxyguanosine(HεdG) were quantitated in gastric mucosa, resected for gastric cancer in Japan and China. The profiles of these DNA adducts identified the origin of the tissues(China or Japan), implying adductome profile may reflect environmental exposures of individual settings. We further tested the repair machinery against these modified bases and discovered TDG play s role in repairing some of these adducts.