Pathological Significance of modified nucleic acid base in human tissue.

人体组织中修饰核酸碱基的病理意义。

• 批准号: 24659161
• 负责人: SUGIMURA Haruhiko
• 金额: $ 2.5万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659161/
• 关键词: DNA 付加体; 修飾核酸; 分子疫学; etheno DNA adduct; demethylation; TET; TDG; IDH; 塩基修復; DNA付加体; DNA修飾; 発がん; 修復遺伝子; 胃がん; 人体病理

Adductomics analysis of alkane substituted exocyclic etheno DNA adducts in human stomach mucosa having gastric cancer was performed for the cases from Lujiang County, Anhui, China, and from Hamamatsu, Japan. Discrimination analysis using quantitative value of 7 lipid per oxidation derived DNA adducts revealed that two regions showed distinctive adductome profile. 3 of 7 DNA adducts in this category were greater in Japanese gastric mucosa than Chinese gastric mucosa, which may suggest gastric carcinogenesis in Japan favor inflammatory or metabolic background. Furthermore, we quantitated 5'-hydroxymethyl deoxycytidine, and its derivatives carboxyl deoxycytidine, and formyl deoxycytidine in gastric cancer and adjacent non-tumor tissues and found the relationship between TET2 expression and 5hmdC. In this attempt we could not identify the adducts specifically high in endemic area of gastric cancer (Lujiang), thus further investigation is necessary.

本文对安徽省庐江县中国和日本滨松市的胃癌患者胃粘膜中的烷基取代外环乙烯基DNA加合物进行了加合分析。对7个氧化产物的定量判别分析表明，有两个区域显示出不同的加合物图谱。日本人胃粘膜中7种DNA加合物中有3种比中国人胃粘膜中的DNA加合物大，提示日本人的胃癌发生更倾向于炎症或代谢背景。此外，我们还对胃癌及癌旁组织中5‘-羟甲基脱氧胞苷及其衍生物羧基脱氧胞苷和甲酰脱氧胞苷进行了定量检测，并发现TET2的表达与5hmdC之间的关系。在这一尝试中，我们无法鉴定出胃癌(庐江)高发区特异高的加合物，因此有必要进行进一步的研究。

项目成果

Internal frontier: the pathophysiology of the small intestine.

内部前沿：小肠的病理生理学。

• DOI: 10.3748/wjg.v19.i2.161
• 发表时间: 2013
• 期刊: World J Gastroenterol.
• 作者: Kurabe N;Sugimura H et al.;Sugimura H and Osawa S
• 通讯作者: Sugimura H and Osawa S

Early-onset diffuse gastric cancer associated with a de novo large genomic deletion of CDH1 gene.

• DOI: 10.1007/s10120-013-0278-2
• 发表时间: 2014-10
• 期刊: GASTRIC CANCER
• 影响因子: 7.4
• 作者: Sugimoto, Shinya;Yamada, Hidetaka;Takahashi, Masazumi;Morohoshi, Yuichi;Yamaguchi, Naotaka;Tsunoda, Yuya;Hayashi, Hiroyuki;Sugimura, Haruhiko;Komatsu, Hirokazu
• 通讯作者: Komatsu, Hirokazu

Towards the genetics and genomics of diffuse gastric cancer

弥漫性胃癌的遗传学和基因组学

• 发表时间: 2013
• 作者: Hiromi Sakamoto;Norihisa Saeki;Hidemi Itoh;Keitaro Matsuo;Hidetaka Yamada;Haruhiko Sugimura;Hitoshi Ichikawa;Hiroki Sasaki;Teruhiko Yoshida
• 通讯作者: Teruhiko Yoshida

DNA Adductome, an ultimate exposome of human tissue.

DNA 加合物组，人体组织的终极暴露组。

• 发表时间: 2012
• 作者: Hiromi Sakamoto;Haruhiko Sugimura et al.;椙村春彦他
• 通讯作者: 椙村春彦他

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关于人类内合组

• 作者: Hiromi Sakamoto;Haruhiko Sugimura et al.;椙村春彦他;Sugimura H;Sugimura H et al.;椙村春彦
• 通讯作者: 椙村春彦