Generation of intestinal epithelial cell line from induced pluripotent stem cell and establishment of colorectal cancer stem cell model

诱导多能干细胞是理想的干细胞，不需要使用胚胎干细胞。本研究首先利用小鼠iPS细胞诱导内胚层祖细胞建立正常肠上皮细胞系，并通过肠道特异性同源盒转录因子CDX2的基因转移分化。下一步，我们的目标是利用大肠癌相关基因的基因转移转化ips诱导的大肠癌干细胞系。通过对这些细胞系的分析，我们旨在通过微阵列分析发现结直肠癌相关基因的新靶点。

Induced pluripotent stem cell is the ideal stem cell and it is not necessary to use embryonic stem cell. In this study, we aimed to establish normal intestinal epithelium cell line by progenitor of endoderm induced from mouse iPS cells in the first step and differentiated by gene transfer of intestine-specific homeobox transcription factor CDX2. In the next step, we aim to generate iPS-induced colorectal cancer stem cell line with transformation using gene transfer of colorectal cancer-related gene. By analyzing the these cell lines, we aimed to identify novel target of the colorectal cancer-related genes with the microarray analysis.