Regulatory mechanisms of osteoclast apoptosis by Bim and Puma

Bcl-2家族蛋白被认为是细胞凋亡的关键调节因子。据报道，在破骨细胞中，Bcl-2家族蛋白不仅调节细胞活力，而且调节骨吸收活性。为了确定促凋亡Bcl-2家族蛋白Bim和Puma的作用，我们制作了基因敲除小鼠并评估骨形态计量学、破骨细胞活力和骨吸收活性。Bim KO小鼠由于骨吸收受损而表现出轻度骨质疏松，尽管它们的破骨细胞存活延长。Puma KO小鼠在骨形态计量学上无表型。为了进一步了解Bim和Puma在破骨细胞中的协同调节作用，需要制备Bim和Puma双基因敲除小鼠（Bim-/-Puma-/-）。

The Bcl-2 family proteins are known as key regulators of apoptosis. In osteoclasts, Bcl-2 family proteins were reported to regulate not only cell viability but also bone resorbing activity. To determine the role of pro-apoptotic Bcl-2 family proteins Bim and Puma, we made knock-out mouse and evaluate bone morphometry, osteoclast viability and bone resorbing activity. Bim KO mice showed mild osteosclerosis due to impaired bone resorption, despite their osteoclast survival were extended. Puma KO mouse had no phenotype in bone morphometry. Puma KO osteoclast had extended viability but there were no significant difference in bone resorbing activity.Making Bim and Puma double knock-out mouse(Bim-/-Puma-/-) are required for further understanding of the co-regulation between Bim and Puma in osteoclast.