Development of technique to disrupt the reproductive function of Monochamus alternatus using an endosymbiotic bacterium, Wolbachia

沃尔巴克氏菌是包括昆虫在内的节肢动物中的一种内共生细菌，能够通过引起细胞质不相容、孤雌生殖、雄性死亡或雌性化来操纵宿主的繁殖。本文研究了人工注射中国粘虫沃尔巴克氏菌(造成寄主细胞质不相容)对松墨天牛的影响。注射沃尔巴克氏菌的个体与未注射的个体的交配试验表明，只有注射的雄性与未注射的雌性交配产生的卵孵化率显着下降。这说明华支睾吸虫的沃尔巴克氏菌对松墨天牛造成了细胞质的不相容。

Wolbachia are endosymbiotic bacteria in arthropods including insects, and capable of manipulating host reproduction by causing cytoplasmic incompartibility, parthenogenesis, male killing or feminization. In this study, effect of artificial injection of the Wolbachia of Collosobruchus chinensis (cause cytoplasmic incompartibility to the host) to Monochamus alternatus was investigated. Mating experiments between Wolbachia-injected individuals and non-injected individuals revealed that only the hatching rate of eggs produced by mating of injected males x non-injected females decreased remarkably. This means that the Wolbachia of C. chinensis caused cytoplasmic incompartibility to M. alternatus.