Development of novel controlling of protozoa without anti-drugs for serving of safety chicken meat

鸡球虫病是由艾美耳球虫原虫寄生虫引起的，主要表现为腹泻。这些寄生虫对全球家禽业造成重大经济损失。我们对线虫胞外产孢和肠道发育阶段进行了全面的基因表达分析，以了解其感染的生物学特性。利用下一代测序仪对cDNA进行测序，发现组装的contigs中含有新的tenella基因。用微阵列分析方法鉴定了鸡盲肠第二代分裂体增殖和分化的上调和下调基因。这些结果表明，所鉴定的基因可能成为控制该病的新靶点，如疫苗抗原。

Chicken coccidiosis is caused by protozoan parasites of Eimeria spp. and mainly characterized as diarrhea. These parasites are responsible for significant economic losses to the worldwide poultry industry. We performed comprehensive gene expression analyses on extracellular sporulation and intestinal developmental stages of E. tenella to understand their biology for the infection. By sequencing of cDNA using the next generation sequencer, assembled contigs were found to contain novel genes of E. tenella. The up- or down- regulated genes at proliferation and differentiation of the second generation schizonts in infected chicken ceca were identified by microarray analysis. These results suggested that the identified genes could be novel targets such as vaccine antigens to control the disease.