Genome-wide exploration of molecular targets for invasion of malignant pleural mesothelioma cell

采用全基因组基因表达分析方法比较人恶性间皮瘤(MPM)细胞及其来源细胞的侵袭能力。在几十个候选基因中，我们关注的是基因X，它在高侵袭性细胞中普遍下调。X基因的敲除加速了亲本细胞的侵袭力，但不改变亲本细胞的增殖和迁移活性。在MPM原位种植模型中，X基因敲除组的存活时间明显短于SCHERBLE组，提示X基因可能是抗MPM侵袭的新靶点基因。

Genome wide gene expression analysis was performed to compare the human malignant mesothelioma(MPM) cells and their derived cells which had higher invasive capacity. Among the dozens of candidate genes, we focused on gene X, which was commonly down-regulated among highly invasive cells. By knocking down of gene X, invasiveness was accelerated, but proliferation and migration activities were not changed in parental cells. In orthotopic implantation model of MPM, the survival was statistically significantly shorter in gene X knock down group than scramble group, suggesting that gene X might be a target gene for a new treatment against the invasion of MPM.