Identification of "cancer stem cell" of mature lymphoid malignancy

为了寻找和鉴定恶性淋巴瘤干细胞，我们使用患者样本用NOG小鼠制作恶性淋巴瘤模型。套细胞淋巴瘤的肿瘤细胞侵犯全身器官，包括肝、脾、骨髓，并在腹腔内形成巨大肿块。从肿瘤中获得的细胞可用于传代至新的NOG小鼠。关于血管免疫母细胞性T细胞淋巴瘤的模型，分析结果显示，患者和NOG小鼠从第一代到第四代的所有肿瘤都是相同的克隆。第二代以后，第一代小鼠体内出现的B细胞、浆细胞和γ球蛋白反应成分消失，肿瘤细胞逐渐增强NOG小鼠体内的致瘤性。

For a search and the identification of malignant lymphoma stem cells, we made the malignant lymphoma model with the NOG mouse using patient samples. The tumor cells of the mantle cell lymphoma invaded the systemic organ including liver, spleen, bone marrow and formed a bulky mass in the abdominal cavity. The cells obtained from the tumor were available for the passage to new NOG mouse. And about the model of the angioimmunoblastic T-cell lymphoma, it was shown that all tumors of the patients and NOG mice from the first to the fourth generation were the same clones as a result of analysis. And B cells, plasma cells and the production of the gamma globulin the reactive component seen with the first generation disappeared the second generation later, and tumor cells were thought to gradually reinforce the tumorigenesity in the NOG mouse in vivo.