Innovative development of antibody drug with ultrastructural in vivo imaging

为了开发廉价、高效的抗体药物，本研究旨在使用具有高空间精度的3D成像来可视化和分析抗体药物在肿瘤组织中的作用部位和作用方式。我进行了体内和体外荧光成像和CT成像，然后旨在整合每个成像数据。我成功地开发了新的体内成像系统，离体成像50μm厚的肿瘤组织，其癌细胞活性良好，和CT成像与新的切片涂层金纳米颗粒。现在，我正在用抗体药物缀合的荧光纳米颗粒或抗体药物缀合的薄片涂层金纳米颗粒使用荧光体内和体外成像或CT成像对荷瘤小鼠进行成像。通过这些研究，初步得出了EPR效应与抗体药效之间的关系。

To develop antibody drug with cheap and high medicinal effect, this study aimed to visualize and analyze where and how the effect of antibody drug works in the tumor tissues using 3D imaging with high spatial accuracy. I performed fluorescence in vivo and ex vivo imaging and CT imaging, and then aimed to integrate each imaging data. I succeeded in developing new in vivo imaging system, ex vivo imaging 50μm thickness tumor tissues whose cancer cells activity were well, and CT-imaging with new slica-coating gold nano-particles. Now I am imaging tumor-bearing mice with antibody drug-conjugated fluorescent nano-particles or antibody drug-conjugated slica-coating gold nano-particles using fluorescence in vivo and ex vivo imaging or CT imaging. As the results of these, I obtained a preliminary result in the relationship between EPR effect and antibody drug effect.