Development and application of novel methods for diagnosis of phytoplankton physiological status

将各种浮游植物生理状态诊断方法应用于人工养殖和天然浮游植物种群，并对其有效性进行了检验。在此过程中，建立了一些海洋微浮游植物和纳米浮游植物的培养。其中，利用荧光底物联合荧光探针ELF-97测定磷酸盐单酯酶和二酯酶活性对浮游植物的限磷性检测非常有效。将该方法应用于南北太平洋，绘制了整个太平洋的磷限量图。从动力学研究中发现，磷酸二酯在热带太平洋地表水中的循环速度比单酯慢得多，这是磷的生物地球化学循环的重要结果。

Various methods to diagnose phytoplankton physiological status were applied to cultured and natural phytoplankton populations and their validity was examined. In this process, some cultures of oceanic pico- and nanophytoplankton were established. Among them, it was confirmed that measurement of phosphate monoesterase and diesterase activities using fluorogenic substrates in combination with a fluorescent probe ELF-97 were very effective in detecting phosphorus limitation of phytoplankton. By applying this method in the North and South Pacific, the map of phosphorus limitation was drawn throughout the Pacific Ocean. From kinetics studies, it was found that phosphate diesters are recycled much slower than monoesters in the surface water of the tropical Pacific, which is an important result for biogeochemical cycles of phosphorus.