Isolation and characterization of prenyltransferases in furanocoumarin biosynthesis

在这项研究中，我们的特点是生物合成基因编码的香豆素特异性异戊烯基转移酶，以了解生物合成的多功能和生物活性的异戊烯基香豆素，在植物中产生的分子机制。我们已经检测到O-和C-异戊烯基转移酶的香豆素底物的微粒体组分从柠檬（柑橘利蒙）果皮，其中大量的异戊烯基香豆素积累。此外，我们还成功地从芸香科植物中分离到了4个香豆素特异性异戊烯基转移酶基因，从伞形科植物中分离到了2个香豆素特异性异戊烯基转移酶基因。

In this study, we characterized the biosynthetic gene encoding to the coumarin-specific prenyltransferases to understand the molecular mechanism underlying the biosynthesis of multifunctional and bioactive prenylated coumarins that are produced in plants. We have detected both O-and C-prenyltransferase activities for coumarin substrates in the microsome fraction prepared from lemon(Citrus limon) peel, where large amounts of prenylated coumarins are accumulated. Moreover, we succeeded to isolate the full length of cDNAs, which would be encoding to coumarin-specific prenyltransferases, four genes from Rutaceae and two genes from Apiaceae.