Microirradiation system for centrosome in human-derived living cells

成功获得了用于活细胞微辐照的全自动三维靶台。因此，我们可以访问细胞碟靶区内的任何细胞，并精确定位细胞内任何点的目标。例如，我们可以添加一种算法来自动识别细胞，或者整合所谓的z-stack图像的能力，这种图像允许对受辐射影响的细胞和区域进行三维表示。系统的首次测试表明，所有组件都完全工作，并且在所有三个维度上都达到了所需的亚微米精度。在台上培养箱中保存数天的活细胞在整个周期内保持健康，细胞周期测量为小于20小时。在第一次固定（不移动）细胞的测试中，我们能够精确地将离子束对准大约1微米宽的中心体，即细胞内的细胞器。改进后的系统将允许精确定位亚细胞成分，并跟踪辐射细胞数天，而不会对细胞产生任何有害影响。

A fully automatic 3-D target stage for the microirradiation of living cells was successfully acquired. We can thus visit any cell within the target zone of a cell dish and position target any point within the cell precisely. We could, for example, add an algorithm to identify cells automatically or integrate the ability to get so called z-stack images which allow a 3-dimensional representation of cells and areas affected by irradiation. First tests of the system show that all components are fully working and that the desired sub-micrometer precision is reached in all three dimensions. Living human cells kept in the on-stage incubator for several days remained healthy over the full period and the cell cycle of the cells was measured as less than 20 hours. In a first test with fixed(not moving) cells we were able to precisely aim the ion beam at the circa 1 micrometer wide centrosome, an organelle within the cells. The improved system will allow precise targeting of sub-cellular components as well as tracking of irradiated cells for several days without any harmful effects on the cells.