Interaction of oxygen carrier protein with mitochondria activity during muscle contraction

细胞呼吸受到多种机制的影响，包括心血管和代谢系统对肌肉中不断变化的能量需求的反应。本研究的重点是肌红蛋白（Mb），一种在骨骼肌和心肌细胞中表达的重要的细胞氧结合蛋白，并提出了Mb与线粒体呼吸直接相互作用的假设。本研究利用啮齿类动物后肢肌肉和培养成肌细胞（L6），获得了Mb直接共定位于肌细胞内线粒体的证据。出乎意料的是，Mb存在于线粒体中。线粒体Mb可与复合体IV相互作用，促进线粒体内的氧消耗。为了获得线粒体Mb在肌细胞中的新的生理功能，需要进一步的研究来阐明线粒体内Mb的靶蛋白。

Cellular respiration is influenced by a wide range of mechanisms including the response of the cardiovascular and metabolic systems to meet the changing energy demands in muscle. The present focused on myoglobin (Mb), an important cellular O2 binding protein that is expressed in skeletal and cardiac muscle cells, and set a hypothesis that Mb will have a direct interaction with the mitochondrial respiration. The present study using rodent hindlimb muscles and cultured myoblast (L6), obtained evidences that Mb directly co-localized on mitochondria within a myocytes. Unexpectedly, Mb presences in mitochondria. The mitochondrial Mb can interact with complex IV to facilitated O2 consumption in mitochondria. Further investigation must be necessary to clarify the target proteins of Mb within mitochondria to obtain the new physiological functions of the mitochondrial Mb in myocytes.