Interaction of myoglobin with mitochondria as an effective oxygen transport system

细胞呼吸受多种机制的影响，包括心血管和代谢系统的反应，以满足肌肉不断变化的能量需求。本研究重点研究了肌红蛋白(Mb)在骨骼肌和心肌细胞中的表达，并假设Mb与肌肉收缩过程中的线粒体呼吸密切相关。利用活体肌肉灌流技术的实验证据表明，Mb在收缩开始时向线粒体释放结合氧，Mb释放氧的反应与线粒体呼吸密切相关。此外，免疫组织化学和免疫沉淀分析表明，Mb的一部分直接共定位于心肌细胞内的线粒体上。

Cellular respiration is influenced by a wide range of mechanisms including the response of the cardiovascular and metabolic systems to meet the changing energy demands in muscle. The present study focused on myoglobin (Mb) expressed in the skeletal muscle and myocardial cells and hypothesized that Mb would closely associate with mitochondrial respiration during muscle contraction. The experimental evidences using in vivo muscle perfusion technique, suggested that Mb released its binding oxygen to mitochondria at onset of contraction and the response of Mb oxygen releasing was closely related with mitochondrial respiration. Furthermore, the immunohistochemical and immunoprecipitate analysis suggested that a part of Mb directly co-localized on mitochondria within a myocyte.