Functional analysis and application of an effective cell-preservation peptide CPP

抗冻蛋白（Antifreeze Protein，AFP）是一种存在于多种生物体内的抗冻蛋白，它能抑制冰晶的生长，从而使生物在寒冷环境中得以生存。我们初步发现一些AFP还具有细胞保存能力，并将其命名为CPP（Cell-Preservation Peptide）。在3年的研究期间，我们对各种AFP的结构-功能关系进行了测试，以获得最有前途的CPP。在2013年，我们终于可以开发出一种方法，通过使用比目鱼衍生的CPP，在+4 ℃下使大鼠胰岛素瘤细胞存活超过5天（PLoS ONE，2013）。此外，我们发表了真菌AFP的第一个晶体结构（PNAS，2012）和基于多维NMR实验的CPP3的结构-功能（JBNMR，2013）。还发表了关于AFP和CPP的生物化学表征和许多应用结果：23篇出版物（包括19篇同行评议文章），40篇演讲（包括14篇特邀演讲）和1项专利申请。

Antifreeze proteins (AFPs) found from many organisms can inhibit ice crystal growth for cold-survival. We preliminarily found that some AFPs also show cell-preservation ability and named them CPP (Cell-Preservation Peptide). During the 3-year research period, we have been tested structure-function relationship of various AFPs to obtain the most promising CPP. In 2013, we finally could develop a method to alive rat insulinoma cells for more than 5 days at +4 degree C by using a flat-fish-derived CPP (PLoS ONE, 2013). In addition, we published the 1st crystal structure of fungal AFP (PNAS, 2012) and structure-function of CPP3 based on the multi-dimensional NMR experiments (JBNMR, 2013). Biochechemical characterizations and many applicational results about AFP and CPP were also published: 23 publications (incl.19 peer-reviewed articles), 40 presentations (incl.14 invited speeches), and 1 patent claim.