Integrated understanding of regulations of DNA replication by cell cycle and chromatin

我们利用模式生物裂殖酵母研究了细胞周期和染色质结构调控DNA复制起始的分子机制。我们发现，磷酸化的多个复制因子，Sld3和Drc1，由细胞周期主激酶CDK是至关重要的复制因子在复制起点触发装配。我们进一步发现了Mcm10在复制因子组装后启动实际DNA合成的作用。在阐明复制定时程序的方法中，我们发现端粒重复序列位于复制晚期的起点附近，端粒结合蛋白参与了复制定时程序。这些研究结果发表在高质量的国际期刊上。

We investigated the molecular mechanisms how initiation of DNA replication is regulated by cell cycle and chromatin structures using model organism fission yeast. We found that phosphorylations of multiple replication factors, Sld3 and Drc1, by cell cycle master kinase CDK are crucial to trigger assembly of replication factors at replication origins. We further discovered the role of Mcm10 after assembly of replication factors to initiate actual DNA synthesis. In approach to elucidate the replication timing program, we discovered that telomere-repeat sequence is located near the late-replicating origins and the telomere-binding proteins are involved in replication timing program. These findings are published on high-quality international journals.