Studies on screening for substances which act on cancer stem cells established from tumors surgically excised from brain tumor patients.

癌症化疗最大的问题之一是获得抗药性。近年来，人们认为“肿瘤干细胞”在肿瘤耐药中起着重要作用。大多数肿瘤起源于肿瘤干细胞，因此被认为是最严重的恶性细胞。因此，肿瘤干细胞是开发抗肿瘤药物的有希望的靶点，但由于肿瘤干细胞不能分化为多种细胞类型，因此很难应用于药物筛选。从癌症患者体内分离出的多形性胶质母细胞瘤(GBM)在球形培养系统中稳定地显示出肿瘤干细胞的特性。在本研究中，我们利用自然文库对作用于GBM细胞的物质进行了筛选。

One of the biggest problems of cancer chemotherapy is the acquisition of drug resistance. Recent years, it has been considered that "cancer stem cell" plays a significant role in drug resistance. Most of tumor is originated from cancer stem cells, thus it is thought to be the severest malignant cells. Like this, cancer stem cells are promising targets for the development of antitumor drugs, however, cancer stem cells are difficult to be applied for drug screenings since it is unstable being to differentiate to various cell types. Glioblastoma multiforme (GBM) excised from cancer patients is stably shows the character of cancer stem cells when cultured in spheroid system. In this study, we performed the screening for substances which act on GBM cells using natural library.