Nutrient sensing and signaling mechanisms of hepatic and intestinal autophagy

自噬是一种普遍存在的细胞内系统，用于降解和回收细胞成分。本研究在大鼠肝癌H4-II-E细胞和胃肠道中研究了自噬的营养调节。特别是，每个氨基酸的调节机制，各种信号机制被证明是依赖于ROS和非依赖性的。精氨酸使用NO途径和mTOR非依赖性。 发现了自噬标志物LC 3分子的mRNA水平以及受氨基酸调控的相关Atg基因。抗氧化食物成分，表没食子儿茶素没食子酸酯和白藜芦醇，刺激自噬。最后，探讨了氨基酸调控自噬与抗衰老基因sirtuins的关系。

Autophagy is a ubiquitous, intracellular system to degrade and recycle cellular components. The nutritional regulation of autophagy was investigated in rat hepatoma H4-II-E cells and gastrointestinal tracts. Especially, the mechanism of regulation, various signaling mechanisms of each amino acid was proved to be ROS-dependent and -independent. Arginine uses NO pathway and mTOR-independent. The level of mRNA of LC3 molecule, an autophagy marker, and related Atg genes regulated by amino acids was discovered. Anti-oxidative food components, epigallocathecin gallate and resveratrol, stimulated autophagy. Finally, the relationship of amino acid regulation of autophagy with sirtuins, an anti-aging gene, were investigated.