Elucidation of diarylheptanpods biosyntheses in achtinorhyzal trees and their actinorhizal symbiosis with Frankia

研究了天南星的甲醇抽提物和环二芳基庚烷类化合物对弗兰克氏菌及其幼苗的影响。结果表明，添加富兰克氏菌的根提取物和环二芳基庚烷类化合物能促进天麻幼苗的生长。和弗兰克娅一起加入了他们的行列。经LC-MS分析证实根提取物中含有环状二芳庚烷类化合物。另一方面，为了鉴定天牛和杨梅的环二芳基庚烷合成酶(CDS)，用下一代测序仪分析了它们的总RNA。根据EST序列和cDNA，构建了假设的全长ORF。这些序列可能编码A.sieboldiana和M.rubra CDS，它们分别暂定为AsCDS和Mr CDS。

The influence of the methanol extractives and cyclic diarylheptanoids of A. sieboldiana on Frankia and the seedling were examined. The results indicated that the growth of A. sieboldiana seedling was enhanced when the root extractives and the cyclic diarylheptanoids were added to them with Frankia. were added to them with Frankia. The presence of cyclic diarylheptanoids in the root extractives was confirmed by LC-MS analyses. On the other hand, to identify the cyclic diarylheptanoid synthases (CDS) of A. sieboldiana and Myrica rubra, total RNAs of them were analyzed by next generation sequencer. From the EST sequences and the cDNAs, hypothetical full-length ORFs were constructed. These sequences might be encoding for A. sieboldiana and M. rubra CDS, and they were tentatively named AsCDS and MrCDS, respectively.