Research on the identification of growth-stimulating peptides from the cerebral ganglion of Pacific abalone

太平洋鲍鱼是东北和北海道地区最重要的水产养殖物种之一。鲍鱼养殖最关键的问题是生长缓慢。为了了解鲍鱼的生长规律，我们尝试测定了鲍鱼的生长刺激肽。利用总RNA构建了鲍鱼脑神经节的cDNA文库，并用大约3300个冒号进行了序列分析。至少检测到42个多肽同源物，其中大量克隆为神经肽Y(NPY)同源物。通过基因克隆和LC-MS/MS检测到两个NPY基因和多肽，分别命名为abNPY-1和abNPY-2。幼鲍注射abNPY后，体质量增加。这些结果表明，NPY同源物存在于大脑神经节中，该肽参与了太平洋鲍体细胞生长的调节。

Pacific abalone is one of the most important aquaculture species in the Tohoku and Hokkaido areas. The most crucial problem for abalone culture is the characteristically slow growth. In an attempt to understand growth regulation in the abalone, we tried to determined growth-stimulating peptide.cDNA libraries of the cerebral ganglions of abalone were prepared using total RNAs, and about 3300 colons were used for the sequence analysis. At least 42 peptide homologs were detected, and large number of clone is Neuropeptide Y (NPY) homolog. Two NPY cDNAs and peptides, named abNPY-1 and 2, were determined by cDNA cloning and LC-MS/MS.Higher AbNPY mRNA levels were observed in the larger-sized abalone than in those of smaller abalone. Injection of abNPY to juvenile abalone resulted in an increase in body mass. These results suggest that NPY homolog is present in the cerebral ganglion and this peptide is involved in the regulation of somatic growth of the Pacific abalone.