Development of rumen microbial profiling to identify low methane-producing cattle

为了建立瘤胃微生物图谱以鉴定低产甲烷牛，研究了抑制甲烷的腰果壳液对瘤胃细菌和古细菌的影响。产甲烷古细菌mOTU35的丰度与荷斯坦干牛、泰国本土牛和沼泽水牛的甲烷产量呈正相关。同时，真细菌OTU95丰度仅与奶牛甲烷产量呈负相关，与丙酸产量呈正相关。这些结果表明，这两个微生物群，尤其是mOTU35，是控制瘤胃甲烷产生的核心成员。因此，对mOTU35的监测可以为估算单牛的甲烷产量提供一个指标。

Toward the development of rumen microbial profiling to identify low methane-producing cattle, rumen bacteria and archaea showing specific changes with methane-inhibiting cashew nut shcll liquid were explored. The abundance of methanogenic archaeal mOTU35 was found to positively correlated to methane production of Holstein dry cows, Thai naitive cattle and swamp buffaloes. Meanwhile, the abundance of eubacterial OTU95 showed negative correlation with methane production and positive correlation with propionate production only in dry cows. These results suggest that both microbial groups, especially mOTU35, is a core member controling methane production in the rumen. Therefore, the monitoring of mOTU35 could offer an index for estimating methane production of individual cattle.