Local distribution of cytosolic calcium ions in activated platelets both on collagen fibrils and on Von Willebrand factor under controlled blood flow conditions.

为了阐明人血小板在胶原纤维或VWF上形成血栓时的初始激活情况，用超快共聚焦激光显微镜实时成像了单个血小板胞浆内钙离子的定位。将单个血小板图像分成16个片段(4×4)，用Image J软件测量每个片段的胞内钙离子浓度([Ca~(2+)]_i)。单个血小板首次与VWF或胶原纤维结合后，[Ca~(2+)]_i一过性升高。在血流状态下，血小板内[Ca~(2+)]_i的升高始于单个血小板的中心分裂，但活化后的血小板内[Ca~(2+)]_i分裂无明显差异。在这项研究中，我们建立了在血流条件下，当血小板与VWF或胶原结合时，在不同激活状态下的血小板钙定位方法。

In order to clarify the initial activation of human platelet in forming thrombi on collagen fibrils or VWF, localizations of intra-cytosolic calcium ion in an individual platelet were imaged in real time using an ultrafast confocal laser microscope. Images of a single platelet were divided into 16 segments (4x4) the intra-cytosolic calcium ion concentrations ([Ca2+]i ) in each segment were measured with software Image J. The [Ca2+]i of individual platelets transiently increased after they first bound to the VWF or the collagen fibrils. The increase in [Ca2+]i started at the center division of each individual platelet under blood flow conditions, although there were no significant difference in [Ca2+]i division in activated platelets. In this study we established the methods of platelet Ca2+ localization under various activated states when platelets bound with VWF or collagen under blood flow conditions.