Development of the method to evaluate the marine ecosystem of mutagen derived from a microbe in the low temperature environment

分析了低温环境下细胞内氧化损伤碱基的合成和积累。分别从房总（6,000 m）和南马里亚纳（2,900 m）区域分离深海细菌，将氧化损伤碱基的解析基因MutT与大肠杆菌来源基因进行比较，MutT的比活性显示出比大肠杆菌高约2倍的高值，并且氧化损伤碱基的底物特异性比大肠杆菌更显著且高。然后，作为在MR 09 -04（JAMSTEC）中收集海水以检查8-OH-G生产的深海中的微生物基因组DNA中的8-OH-G量的结果，深海微生物比来自表面海水的微生物多约10倍。

We analyzed the synthesis and accumulation of oxidative damage base in the cell in low temperature environment. After isolating deep sea bacteria each from the Boso ( 6,000m) and south mariana area ( 2,900m), and comparing resolution gene MutT of the oxidative damage base with the Escherichia coli origin gene, the specific activity of MutT showed a high value of approximately 2 times than Escherichia coli and the substrate specificity for the oxidation damage base was more remarkable than Escherichia coli and was high. Then, as a result of 8-OH-G quantity in microbe genomic DNA in the deep sea which collected sea water in MR09-04 (JAMSTEC) to check 8-OH-G production, there were more deep sea microbes approximately 10 times than an microbe from surface sea water.