Assessing the balance of genotoxic and protective effects by flavonoids in human cells and development of a novel protein nanochip to monitor protection

多酚可能是健康的食品成分，因为它们具有抗氧化活性，并具有调节I期和II期酶的特性，从而导致细胞化学耐药性。然而，在饮用含有花青素或儿茶素的果汁的人的血液淋巴细胞中，我们首先观察到谷胱甘肽s -转移p的表达减少，随后诱导该蛋白。这意味着由于果汁的消耗，有一个最初的漏洞，随后是增强的保护。显然，有必要了解这些对比效应对人类的影响。因此，我们将对黄酮类化合物在人类淋巴细胞中的积极和消极影响进行动力学机制研究，并开发一种基于抗体的纳米芯片，以量化选定的II期蛋白的表达，用于生物标志物研究。为此，我们将研究两种多酚混合物如何调节生物转化系统。我们将测定谷胱甘肽s -转移酶（GSTs）、醌还原酶（QR）或udp -葡萄糖醛基转移酶（UGT）的表达水平，作为细胞解毒能力的功能标志物，并比较多酚治疗前后的基线水平。

Polyphenols are probably healthy food ingredients on account of their antioxidant activities and their properties to modulate phase I and II enzymes in way that results in cellular chemoresistance. In blood lymphocytes from humans consuming juices with anthocyanins or catechins, however, we first observed a decreased expression of glutathione S-transferate p, followed by an induction of the protein. This implies that there is an inital vulnerability followed by enhanced protection due to the juice consumption. Obviously, it is necessary to understand the consequences of these contrasting effects in humans.Therefore, we will perform kinetic mechanistic studies on positive and negative impacts of flavonoids in human lymphocytes and develop an antibody-based nanochip to quantify expression of selected phase II proteins for biomarker studies. For this we will investigate how two mixtures of polyphenols modulate the biotransformation system. We will determine the expression levels of glutathione S-transferases (GSTs), quinone reductase (QR) or UDP-glucuronosyl-transferase (UGT), as functional markers of the cells' detoxification capacities and compare baseline levels before and after treatment with polyphenols.