Molecular identification and characterisation of loci causing heterosis in Arabidopsis thaliana

本项目旨在阐明拟南芥（Arabidopsis thaliana）植物模式系统中杂种优势（杂种优于亲本）的分子基础。为此，将研究两个不同的材料，C24和Col，它们的F1杂交种显示出杂交活力。通过对两个亲本、它们的F1杂交种、约400个衍生基因型重组自交系（RILs）和它们的测试交杂交种的分析，将确定影响植物发育早期生长（生物量、叶面积）的杂种优势数量性状位点（hQTL）。本项目将使用相同的遗传物质进行基因表达谱实验（如发育后期生物量和叶面积分析、蛋白质谱、代谢物谱和幼苗建立期间的生理和发育过程），以深入了解与杂种优势相关的分子过程。

The proposed project aims at the elucidation of the molecular basis of heterosis (the superior performance of hybrids as compared to their parents) in the plant model system Arabidopsis thaliana. To this end, two divergent accessions, C24 and Col, will be studied, the F1 hybrids of which were shown to exhibit hybrid vigour. Through the analysis of the two parent lines, their F1 hybrids, ca. 400 derived genotyped recombinant inbred lines (RILs) and their testcross hybrids, heterotic quantitative trait loci (hQTL) will be identified that affect growth (biomass, leaf area) at early stages of plant development. Gene expression profiling experiments will be performed in this program (such as the analysis of biomass and leaf area at later stages of development, protein profiles, metabolite profiles and physiological and developmental processes during seedling etablishmend) that use the same genetic material in order to gain in-depth insight into the molecular processes associated with heterosis.