Mechanism of anesthetic action studied by interfacial vibrational spectroscopy

通过测量硬脂酸钠水溶液、氩气、纯水和氩气流中的SFG光谱，研究了硬脂酸钠分子在熔融石英/溶液界面上的吸附行为。强峰由于CH伸缩的CH 3基团出现在氩气流下，显示硬脂酸单分子层的存在下，具有非常高的构象顺序。而与水接触时，没有检测到SFG信号，表明硬脂酸单分子膜在水中的结构是完全无序的。实验还发现，只有在低于临界胶束浓度（CMC）时，才能形成有序的硬脂酸单分子层。通过SFG和AFM测量研究了海藻糖对DMPC双分子层干态稳定性的影响。发现海藻糖在干燥状态下对DMPC双分子膜的稳定是有效的。结果表明，海藻糖在干燥状态下对DMPC双分子层结构域的稳定是有效的。

Adsorption behavior of sodium stearate molecules at fused quartz/solution interface was studied by measuring SFG spectra in sodium stearate aqueous solution, followed by under Ar gas, in pure water, and under Ar gas flow again. Strong peaks due to CH stretching of CH3 group appeared under Ar gas flow, showing the presence of the stearic acid monolayer with very high conformational order. However, no SFG signal was detected when it was contact with water, showing that the structure of stearic acid monolayer was totally disordered in water. It was also found that the well ordered stearic acid monolayer was formed only below critical micelle concentration (CMC).The effect of trehalose on the stability of DMPC bilayer in dry state was also studied by SFG and AFM measurements. It was found that trehalose was effective for stabilizing DMPC bilayer in dry state. Results showed that trehalose was effective for stabilizing DMPC bilayer domain in dry state.