The improvement effect of xenon gas on cellular viability for cryopreservation of biological tissue

本课题的目的是阐明氙气的有效性，以减少冷冻过程中细胞间应力造成的损害和解冻条件下低温本身对细胞的损害，以降低具有较厚生物组织水平的样品的冷冻保存。首先，考察了二甲基亚砜作为典型的冷冻保护剂与氙气的加压溶解减压相结合，在细胞冷冻过程中减少细胞损伤的增强效果。检测了4℃低温胁迫对细胞的保护作用。结果表明，当样品以较低的降温速率冷冻时，氙气的溶解对减少细胞间应力的损伤是有效的，而加压氙气在非冷冻条件下可以有效地减少低温本身对细胞的损伤。

The purpose of this task was to clarify the efficiency of a xenon gas in order to reduce the damage by the intercellular stress during freezing and the cellular damage by the low temperature itself under an unfrozen condition in cryopreservation of a sample with a level of thick biological tissue. First, the enhanced effect of reduction of cellular damage in freezing process of a cell with a combination of dimethylsulfoxide as a typical cryoprotectant and pressurized dissolution and decompression of xenon gas was examined. And then the protective effect to the cell against the stress of the low temperature itself at 4 degree Celsius was examined. As a result, when a sample was frozen at a low cooling rate, it was suggested that dissolution of xenon gas was effective in reduction of damage by the intercellular stress, and it was clear that pressurized addition of xenon gas was effective in reduction of the cellular damage by the low temperature itself under an unfrozen condition.