Development of basic glycotechnology for construction of multifunctional proteoglycan molecules.

研究了牛睾丸透明质酸酶和黑木耳纤维素酶的水解和转糖基化反应，以期将其应用于蛋白聚糖的糖化工艺。作为反应底物，除了蛋白聚糖外，还使用了市售的糖链，以及在核心肽和蛋白聚糖糖链之间具有连接结构的低聚糖，以及我们制备的脱硫酸糖链。我们发现了一种被透明质酸酶降解的糖卡因结构，但可能不作为转糖基化反应的供体底物。确定了纤维素酶水解蛋白多糖的最佳条件，但未检测到转糖基化反应。

Hydrolysis and transglycosylation reactions of hyaluronidase (from bovine testes) and cellulase (from A. nigar) were investigated with the aim of applying them in glycotechnology for proteoglycans. As reaction substrates, commercially available sugar chains in addition to proteoglycans, as well as oligosaccharides having linkage structures between a core peptide and a sugar chain of proteoglycan, and de-sulfated sugar chains that we prepared, were used. We found a sugar cahin structure that was degraded by hyaluronidase but possibly did not act as a donor substrate for the transglycosylation reaction. The optimal condition for proteoglycan hydrolysis by cellulase was determined, however, transglycosylation reaction could not be detected.