Mechanism of the development of local organizers in the brain primordium using zebrafish as a model system

我们研究了斑马鱼脊椎动物脑形成的调控机制。我们揭示了两个峡部形成基因gbx2和pou2的功能细节，表明它们都参与了神经发生。我们还扩展了anb形成基因emx3和峡部形成基因pax2a和pou2的转录调控分析。同时，神经嵴和体突的发育受一种新的突变kanazutsi的影响，其致病基因编码一种球蛋白家族蛋白。此外，我们成功地引入了两种新的实验技术，GAL4/UAS系统和TALEN。

We studied the regulatory mechanism of vertebrate brain formation in zebrafish. We revealed the details of the function of two isthmus-forming genes, gbx2 and pou2, suggesting that both are involved in neurogenesis. We also extended the analysis on the transcriptional regulation of an ANB-forming gene, emx3, and two isthmus-forming genes, pax2a and pou2. Meanwhile, it was shown that the development of the neural crest and somite is affected in a novel mutation, kanazutsi, and its causative gene encodes a globin-family protein. In addition, we succeeded in introducing two novel experimental techniques, the GAL4/UAS system and TALEN.