Regulatory mechanisms in pupal color development of some butterflies

通过C18-HPLC使用卡氏弧菌的结扎的质粒将OPIF样活性分级分离为一个峰。尽管OPIF的一级结构尚未确定，但可以确定OPIF的纯化步骤。为了了解4种蝴蝶蛹色的调控机制，研究了环境因素对滞育和/或非滞育蛹色的影响。在P. machaon中，至少有两种产生棕白色脓的激素因子位于幼虫的中枢神经系统中，这些因子的分泌受到长日幼虫对环境线索的识别的调节。在B。影响滞育蛹色的主要因素是消化后的温度和湿度。在阿宝。c-aureum的蛹色多型性主要受温度和少量光周期的共同调节。绿色腐皮主要由幼虫食物气味和较高的相对湿度产生。

OPIF-like activity was fractionated as one peak by C18-HPLC using ligatured abdomens of V. cardui pharate pupae. Purification steps for OPIF may be decided although the primary structure of OPIF was not determined. To understand the regulatory mechanism underlying the control of pupal coloration in 4 butterflies, the effect of environmental cues on diapause and/or non-diapause pupal coloration is investigated. In P. machaon, at least two hormonal factors producing brown-white pupae are located in the larval central nervous system, with the secretion of these factors being regulated by the recognition of environmental cues in long-day larvae. In B. alcinous, temperature and humidity experienced after a gut purge are the main factors that affect the diapause pupal coloration. In Po. c-aureum, the pupal color polyphenism is co-regulated by mainly temperature and a small degree photoperiod. Green pupae of P. bianor were mainly produced by a larval food smell and highly relative humidity.