Clarification of mechanism of biofilm formation and degradation of toxic compounds by genome sequencing and numerical analysis

本文主要研究了降解芳香族化合物的细菌生物膜的形成。测定了PCB降解菌Comamonas testosterone - steroni TK102的基因组，分析了降解和生物膜形成过程中的特异性表达。我们还进行了生物膜生长模拟。联苯羟基化合物的加入促进了生物膜的形成和活细胞的数量。利用下一代测序系统对菌株TK102进行全基因组测序，将PCB降解基因编码在一个移动遗传元件上。数值结果表明，生物膜种子的初始位置是影响种子生长的重要因素，种子在生物膜上的对流流动与养分向生物膜扩散的相对关系影响种子的生长行为。

This research was focused on the biofilm formation of bacteria which degrade aromatic compounds. Genome of a PCB degrader Comamonas testosteroni TK102 was determined for the analysis of specific expression during degradation and biofilm formation. We also carried out a biofilm growth simulation. Addition of hydroxyl compound of biphenyl enhanced biofilm formation and number of live cells. Whole genome sequence of strain TK102 was done by next generation sequencing system and PCB degradation genes were coded on a mobile genetic element. From numerical results, the initial position of biofilm seeds proved to be an important factor and growth behavior was influenced by the relative relationship between convection of flow over biofilm and nutrient diffusion into biofilm.