Functional analysis of novel terpene cyclases that resemble transporter

吡喃是一种由真菌烟曲霉产生的化合物，是一种有望作为降胆固醇药物开发的硫代萜类化合物。在吡喃生物合成基因簇中，发现了一种新的类似于转运蛋白的萜烯环化酶。本研究对其活性部位残留物进行了详细的分析。此外，还从烟曲霉和引起赤霉病的禾谷镰刀菌中鉴定出第二个分枝萜类生物合成基因簇。烟曲霉菌中第二个基因簇中的PKS产生TAL。

Pyripyropene, produced by a fungus Aspergillus fumigatus, is a meroterpenoid that is expected to be developed as a cholesterol lowering drug. Among the pyripyropene biosynthetic gene cluster, a novel terpene cyclase was identified that resembled more like a transporter. In this study, detailed analysis of its active site residue was carried out. Besides, a second meroterpenoid biosynthetic gene cluster was identified from A. fumigatus as well as from a fungus Fusarium graminearum, which causes Fusarium head blight. The PKS among the second gene cluster in A. fumigatus was shown to produce TAL.