Mass production of 15N labeled DNJ to evaluate the absorption and excretion of 1-deoxynojirimycin

桑叶中含有独特的1-脱氧诺吉霉素，能抑制餐后血糖，因此被认为是有望预防糖尿病的食材。DNJ作为功能性食品材料要被市场广泛接受，必须进行充分的安全性评价。在本研究中，我们试图阐明DNJ的吸收、分布、代谢和排泄。我们利用发酵技术建立了DNJ的批量生产方法，并成功生产了纯化的15N标记DNJ 10g。我们给大鼠口服纯化的15N标记的DNJ，目前正在进行机制分析和安全性评价。

Mulberry leaves have the unique ingredient called 1-deoxynojirimycin which suppresses the postprandial blood glucose, so that are considered as the food materials which are expected to prevent diabetes. Sufficient safety assessment of DNJ is necessary to be widely accepted as the functional food material in the market. In this research, we tried to clarify the absorption, distribution, metabolism and excretion of DNJ. We established the mass production method of DNJ by using fermentation technique, followed by succeed to produce 10g of the purified 15N labeled DNJ. We orally administrated the purified 15N labeled DNJ to rats, now studying the mechanism analysis and safety evaluation.