Identification of lesions responsible for fatal outcome in rats infected with Borna disease virus

新生大鼠脑内接种重型博尔纳病病毒（BDV） CRNP5变体、轻型博尔纳病病毒（BDV） CRP3变体和9种重组博尔纳病病毒（rBDV）变体，观察8周。1. CRNP5在博纳病（BD）的发病率和严重程度上得分最高。四组rBDV变体的评分高于CRP3。2. 从接种后第20天开始，4个rBDV变异体的体重增长均低于其他rBDV变异体和CRP3。3. 接种BDV的脑组织抗BDV抗体免疫组化阳性。死亡幼犬的病毒滴度明显高于终止幼犬。4. 包括L1（L1417R）突变在内的4种rBDV变异对单个核细胞血管周割裂的严重程度评分较高，L1（L1417R）可能在BD的致病性中起重要作用。脑膜脑炎仅在rBDV变异中观察到。死亡的小狗得分更高。

Newborn rats were inoculated intracranially with CRNP5 variant of Borna disease virus (BDV) with severe pathogenesis, CRP3 BDV variant with mild pathogenesis, and nine recombinant BDV (rBDV) variants and examined for 8 weeks. 1. The CRNP5 had the highest score for incidence and severity of Borna disease (BD). Four groups of rBDV variants had a higher score than CRP3. 2. The body weight increase of four rBDV variants was lower than that of other rBDV variants and CRP3 from day 20 after inoculation. 3. All BDV inoculated brains were positive for anti BDV antibodies immunohistochemically. The virus titer for pup fatalities was significantly higher than that of pups on termination. 4. Four rBDV variants including L1(L1417R) mutation had higher scores for the severity of perivascular cuffing of mononuclear cells and L1(L1417R) may play an important role for the pathogenicity of BD. 5. Menigoencephalitis was observed only in rBDV variants. The pup fatalities had higher scores.