Establishment of the disinfection technique with hydroxyl radicals and catalase inhibitor based on the reaction kinetic analysis

过氧化氢光解消毒处理可能是现有抗生素的一种新的替代方法，因为该消毒处理引起细菌耐药性的风险被证明是相当低的。此外，我们还发现，在含有多酚的中草药和植物提取物的存在下，光照射的过氧化氢的杀菌活性被协同增强。消毒处理的杀菌活性是由于过氧化氢光解产生的羟基自由基，这些自由基在停止光照射后立即消失。因此，残留毒性不会有问题，而且中草药和植物提取物的抗氧化活性将减轻光照射过程中可能由羟基自由基造成的氧化损伤。

Disinfection treatment with photolysis of hydrogen peroxide could be a novel alternative to existing antibiotics because the risk of inducing bacterial resistance by the disinfection treatment was proven to be quite low. In addition, we revealed that bactericidal activity of photo-irradiated hydrogen peroxide was synergistically augmented in the presence of polyphenol containing-herbal medicines and -plant extracts. The bactericidal activity of the disinfection treatment is due to hydroxyl radicals generated by photolysis of hydrogen peroxide which disappear immediately after the cessation of photo-irradiation. Therefore, there would be no problematic issues on residual toxicity, and there would be an advantage that antioxidant activity of the herbal medicines and plant extracts would alleviate the oxidative damage that might be caused by hydroxyl radicals during photo-irradiation.