Biopharmaceutical Study on Organic Solute Transporters Which Modulate Central Nervous System

氨基酸和神经递质在大脑中的体内命运是通过神经元和/或星形胶质细胞的释放和它们的再摄取来精确调节的，并负责维持大脑的高阶功能。在本研究中，我们研究了n -乙酰- l-天冬氨酸- l-谷氨酸（NAAG）和肉碱转运体在小鼠初代培养的神经元和星形胶质细胞中的功能特征。在星形胶质细胞中发现NAAG被寡肽转运体（PEPT） 2所吸收。此外，还发现神经元中氨基丁酸转运体（GAT） 1和肉碱/有机阳离子转运体（OCTN） 2摄取肉碱。

In vivo fate of amino acids and neurotransmitters in brain is precisely regulated by release from neuron and/or astrocytes and by their re-uptake, and is responsible for maintenance of brain high-order function. In this study, we investigated the functional characterization of N-acetyl-L-aspartyl-L-glutamate (NAAG) and carnitine transporter in mouse preimarily cultured neurons and astrocytes. NAAG was found to be taken up by oligopeptide transporter (PEPT) 2 in astrocytes. In addition, carnitine was found to be taken up by GABA transporter (GAT) 1 and carnitine/organic cation transporter (OCTN) 2 in neuron.