Function of novel BRCA2-associating proteins in centrosomal regulation and DNA repair

BRCA2种系突变是遗传性乳腺癌和卵巢癌的主要原因。BRCA2的一个主要功能被认为是DNA损伤修复中同源重组的调节因子。此外，BRCA2在中心体调控中也起着重要作用，其功能障碍可能参与了乳腺癌的发生。即便如此，详细的分子机制还没有被发现。在目前的研究中，我们试图定位BRCA2中负责这一功能的分子区域。一系列BRCA2片段在人培养细胞中外源性表达。计算细胞中中心体的数量，以观察特定片段的过度表达是否会损害中心体的数量完整性。为此，我们开发了一种基于图像识别的自动中心体计数系统，使我们能够在没有人为偏见的情况下判断大量转染的细胞。

BRCA2 germline mutations account for the majority of heredity breast and ovarian cancer. A major function of BRCA2 is known as a regulator of homologous recombination in DNA damage repair. In addition, BRCA2 also plays an important role in centrosomal regulation, whose dysfunction might be involved inthe tumorigenesis of breast cancer. Even so, detail molecular mechanism isnot yet uncovered. In the present study, we tried to locate a molecular region of BRCA2 responsible for this function. Series of BRCA2 fragments were exogenously expressed in human cultured cells. Numbers of centrosome in a cell are counted to see if an overexpression of a specific fragment could impair a numerical integrity of centrosomes. For this purpose we developed an automated centrosome-counting system based on image recognition, which allows us to judge a large number of transfected cells without human bias.