Identification of Pancreatic Cancer Stem Cells and Development of Novel Therapeutic Strategies using Stem Cell Markers

人胰腺导管腺癌的肿瘤干细胞标记物在癌前病变、Panin和IPMN中表达。在癌前病变中，高异型增生的表达水平明显升高，在腺癌中明显过表达。CD133在腺癌中的过度表达与静脉侵犯有关。CD24、ESA和CXCR4在腺癌中的表达与高分化类型有关。细胞增殖标记物的Ki-67和MCM2标记指数在高级别癌前病变和低度癌前病变中较大，在原位癌和浸润性病变中显著增加。我们建立的新的导管腺癌细胞系CXCR4过表达。该细胞株和2株腺鳞癌细胞株均高表达ESA和MCM2。这些细胞系在裸鼠体内不具有致瘤性。

The cancer stem cell markers for human pancreatic ductal adenocarcinoma are expressed in precancerous lesions, PanIN and IPMNs. The expression levels had increased strongly higher dysplasia in the precancerous lesions and marked overexpression was observed in adenocarcinomas. The CD133 overexpression in adenocarcinoma correlated with venous invasion. CD24, ESA and CXCR4 expressions in adenocarcinomas were related with well-differentiated types. The Ki-67 and MCM2 labeling indices of the cell proliferation markers was greater in higher grade precancerous lesions compered to low grade precancerous lesions, and increased markedly in carcinoma in situ and in invasive lesions. The newly ductal adenocarcinoma cell line that we established showed an overexpression of CXCR4. This cell line and 2 adenosquamous cell lines showed high levels of expression of ESA and MCM2. These cell lines were not tumorgenic in nude mice.