Searching for neurodegenerative disease-related factors in the metabolic systems of ADMA-containing protein and the elucidation of the functional mechanism

为阐明不对称二甲基精氨酸（ADMA）代谢途径在中枢神经系统的生理意义，我们采用免疫组织化学方法检测了ADMA代谢酶PRMT 1、DDAH 1和nNOS在大鼠脊髓和其他脑区的分布。Western blot分析表明PRMT 1和DDAH 1在大肠杆菌中分布广泛。免疫组织化学分析表明，这些酶共定位于许多神经元。在皮层和下丘脑，几乎所有的nNOS神经元都有PRMT 1和DDAH 1。此外，我们调查了未知的精氨酸甲基化蛋白质从大鼠脑蛋白质组学分析，并确定果糖1，6-二磷酸醛缩酶C（FBAC）作为一种新的含ADMA的蛋白质。提示PRMT/DDAH/NOS轴在中枢神经系统内起着一定的稳态调节作用。

To demonstrate the physiological significance of the metabolic pathways for asymmetric dimethylarginine (ADMA) in central nervous system, we examined the immunohistochemical localization of ADMA metabolizing enzymes, PRMT1 and DDAH1 and nNOS in the rat spinal cord and the other brain regions. Western blot analyses showed that PRMT1 and DDAH1 were widely distributed. The immunohistochemical analyses showed that these enzymes colocalized in a lot of neurons. In the cortex and hypothalamus, almost all nNOS neurons had PRMT1 and DDAH1. Additionally, we surveyed unknown arginine methylated proteins from rat brain by proteomic analysis and identified fructose 1,6-bisphosphate aldolase C (FBAC) as a novel ADMA-containing protein. These findings suggest that the PRMT/DDAH/NOS axis is play a part of homeostatic function in central nervous system.