Pathophysiological correlation between fibrogenesis and regeneration in murine liver via Notch/Jagged-1 signal

为了阐明Notch信号参与肝纤维化发生和再生之间的相关性，通过肝纤维化的蛋氨酸/胆碱缺乏饮食（MCD）模型检查Notch信号的表达。在MCD饲料开始后2、4和8周处死小鼠。通过免疫印迹研究Notch受体和Notch配体的肝脏表达。MCD组与对照组在肝纤维化过程中Notch信号的表达无显著性差异。在我们使用条件性Mx/Cre Jagged-1敲除小鼠的初步数据中，推测Notch信号调节肝纤维化中纤维发生和再生之间的相关性。而在MCD模型中，Notch信号不参与肝纤维化。通过使用其他肝纤维化模型，进一步的研究是必要的，以阐明Notch信号在肝纤维化过程中的作用。

To clarify the involvement of Notch signal in the correlation between fibrogenesis and regeneration in the liver, expression of Notch signal was examined by methionine/choline-deficient diet (MCD) model of liver fibrosis. Mice were sacrificed at 2, 4, and 8 weeks after MCD diet started. Hepatic expression of Notch receptors and Notch ligands were investigated by immunoblot. There were no significant differences between MCD group and control group in the expression of Notch signal during the progression of liver fibrosis. In our preliminary data by using conditional Mx/Cre Jagged-1 knockout mice, it was speculated that Notch signal modulates the correlation between fbrogenesis and regeneration in liver fibrosis. However, in MCD model, Notch signal was not involved in hepatic fibogrnesis. By using other liver fibrosis models, further studies are necessary for the elucidation of the role of Notch signal in the fibrogenic process in the liver.